Vidro was out of the lineup after playing first base in Monday night's marathon 13-12 loss in 10 innings to the Rangers.
"It wasn't a good idea," Seattle manager John McLaren said. "He's sore today."
McLaren said Vidro will be re-evaluated when the Mariners get back home for Friday's first Interleague series against the Padres. That means Vidro likely will miss Wednesday afternoon's game in Texas. Vidro took treatment for the back spasms before Tuesday's game.
Vidro's occasional appearances in the lineup have hindered his efforts to get out of a season-long slump. He was 1-for-6 in Monday's game, dropping his average to .193.
Vidro is batting .105 in May, but he's only played in five of Seattle's 13 games this month. The injury has precluded him from finding any kind of consistency at the plate.
"We need to rest him," McLaren said. "Then we'll look at him when we get back home."
What's worse for the Mariners is Vidro was being counted to bat fifth in the lineup, behind cleanup hitter Adrian Beltre. That spot has been turned over to Jose Lopez, who is 7-for-12 since hitting fifth, but the Mariners still need a healthy Vidro, who hit .314 with 59 RBIs last season.
Seattle's offense has slumped without Vidro hitting in the middle of the lineup and with the struggles of Kenji Johjima and Richie Sexson.
The Mariners went through a stretch where they averaged 1.78 runs in the first nine games of May, and they lost all but one of them. Seattle scored 22 runs and batted .333 in its last three games, but the Mariners lost twice.
"We're fighting, and it was great when Kenji hit the home run [in the ninth inning of Monday's game]," McLaren said. "We've kind of been on a roller coaster."
Todd Wills is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.